Thursday, December 8, 2016

Brain Anatomy Lab Narrative


                                                             Brain Anatomy Lab
Description: For this lab we were given a cauliflower that represented a brain. We first had to research then label each part of the brain onto the piece of cauliflower. Once all pieces were labeled Mrs. Neto gave our group the situation of our cauliflower brain having a stroke in the left frontal lobe. After giving us the situation we were told to write a narrative as the patient, doctor, or family member of the patient. I chose to write my narrative as the child of the patient that suffered from the stroke.

Narrative
I should have been more observant and cautious when it came to my mom’s health. After the doctor had explained what the symptoms are for an oncoming stroke, I knew that I could have tried to prevent it, or at least received  help earlier. With a history of health problems such as high blood pressure and a heart attack that had only occurred a year earlier, my mom was a likely candidate for a stroke in her left frontal lobe, especially because she had been so caught up with trying to keep a roof over our heads, that taking care of herself was not one of her top priorities.
Mom had been complaining of having trouble seeing through her left eye, and numbness in her arms and legs a few days earlier. It worried me because she did not usually complain unless something had been really bothering her, but when I had asked her if she needed to go to the doctor, she brushed me off saying that she would be fine and that she must have only been tired. Two days later, when I went to wake her to start getting ready for work, she started to speak in a very quiet, non- comprehensible manner. I tried to help her get out of bed only to have her crash to the floor. Her body became completely stiff as she layed on the ground slowly becoming unconscious, while still trying to speak through her slurred words.
By the time I had reached the hospital the doctor was already prepared to speak to me, he explained that my mom had just had a stroke in the left frontal lobe of her brain, “You are both lucky you found her when you did, there was a significant amount of bleeding, if you would have waited any longer she would have died.” If only the doctor knew that the mom I had grown up with, and loved so dearly, had actually died that day. She was never the same after her stroke, and  I will never forget the first words she spoke to me after waking up, “Do I know you?” My heart broke in that moment, but the doctor was quick to try and comfort me, “I know it’s hard but memory loss is very common with stroke patients. I want you to be prepared to help her deal with the long-term effects of a stroke, such as a choppy speech pattern, weakness in the left side of her body, and changes in her behaviour and personality.” I was prepared to do whatever it took to nurse my mom back to health, even if it meant dropping out of school and getting a job to be able to pay the bills and keep her healthy.
After a two week hospital stay, my mom was released. During those two weeks I had watched the one person I once felt so connected to, become someone completely unrecognizable, and I now had to take this stranger home with me. She was my mom and I still loved her more than anything in the world, but no amount of information or help from the doctors had prepared me to take care of her. She became extremely antisocial, some days she would not even look at me. I would wake up in the middle of the night to her cries, and without thinking I would run to her room but she would not accept my help. I could tell she was constantly in pain, it hurt me so much to see the one person that mattered so much to me in so much pain.  She was suffering, and I felt so helpless. She did have good days though, we would sit and watch movies and laugh until we cried, but those days were rare. She was starting to complain of headaches, but when I asked the doctor about them, he had said she would be fine and that they were very common. I guess it was common for stroke patients to have another stroke too, because that is what happened to my mother. This time I didn’t get to her on time though. My mom died only two months after her first stroke. The only thing I have left in life is hope, I have hope that she is in a better place and no longer suffering.




Wednesday, November 16, 2016

Iron Chef Lab

Iron chef lab - 
  For this lab, my Iron Chef group chose strawberries from the mystery box. With our food chosen, we decided to see if conventional or organic strawberries would grow more mold throughout the length of the experiment. We swabbed the strawberries and put it on the agar that we had previously made. With the help of the agar and the incubator, we were able to grow mold. The end goal of the project was to see which type of strawberry would grow a certain mold called botryis dextrin.

Procedures:
  1. Make Regular Agar and pour into 6 petri dishes.
  2. Make PDA and pour into 6 different petri dishes
  3. Refrigerate for 3 days
  4. Swab the outside of organic strawberries and place into 2 PDA dishes and 2 RA dishes
  5. Swab the outside of conventional strawberries and place into 2 PDA and 2 RA dishes
  6. Leave 2 RA dishes and 2 PDA dishes alone as a control
  7. Put all 12 dishes into an incubator
  8. After 1 week of incubation Take out 1 dish from each group (conventional pda, control pda, organic pda, conventional ra, control ra, organic ra) and use the ocular loop to scrape off a sample from each dish.
  9. Transfer these samples to individual slides and cover them with slide covers.
  10. Place the slides under microscopes and examine them for botrytis cinerea.
  11. If it is a positive id then mark it as such in the data table.
  12. Repeat steps 7-10 at  1.5  weeks for the other set of petri dish samples.
  13. Repeat steps 7-10 again at 2 weeks for the first set of petri dishes.
  14. Repeat steps 7-10 again at 2.5 weeks for the second set of petri dishes.
Materials:
  • 1 box of strawberries (organic)
  • 1 box of strawberries (non organic)
  • Potato Dextrose agar
  • Basic Agar
  • Petri dishes
  • Microscope
  • Slides
  • Slide covers
  • Swab
  • Iodine  (staining as needed)
  • Incubator
  • Ocular Loop
Skills


  • Fungus identification
  • Microscope usage
  • Incubator usage
  • Agar plate making

Hypothesis-

If a lab technician swabs a organic and a conventional strawberry and transfers it into in an agar plate and then an incubator then the organic strawberries’ agar plate will grow more botrytis cinerea than the agar plate with the swab from the conventional strawberry because the organic strawberries are not allowed to use pesticides to prevent mold.